AC010761.14

Species: Homo sapiens

Position: chr17: 28727257-28727739

Known as:

Transcript: ENST00000587898

Sequence: Download

Description:

We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). We classified lncRNA genes as hits if their combined phenotype effect size and P value (referred to here as “screen score”) exceeded a consistent threshold applied to each screen corresponding to an empirical FDR of 5%. Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth modifying function exclusively in one cell type.



sgRNAs

sgRNA_ID Sequence Position (Chr) Position (Lnc) Length PAM Type Validity Cell line Note Ref.
sgRNA1 GGTGAGAACACGGGTGTCCT 28727355-28727374(+) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA2 GTGTAACCTTGGAGCCATAG 28727286-28727305(-) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA3 CAGCTCTGTGAGCCAAGGGG 28727398-28727417(-) gene body (near 5') 20 AGG CRISPRi Partial validated iPSC NA [1]
sgRNA4 AGCCAGCGAGGGAAGTTAAG 28727113-28727132(+) up stream 20 CGG CRISPRi Partial validated iPSC NA [1]
sgRNA5 CCTTGCGAGAGCCAGGACAG 28727492-28727511(+) gene body (near 3') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA6 GGAGAAGAGGGTAGTAATAG 28727152-28727171(+) up stream 20 AGG CRISPRi Partial validated iPSC NA [1]
sgRNA7 AAGCGGAGATGAGAGCGGGG 28727130-28727149(+) up stream 20 AGG CRISPRi Partial validated iPSC NA [1]
sgRNA8 AGTTAAGCGGAGATGAGAGC 28727126-28727145(+) up stream 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA9 AGAGGGTAGTAATAGAGGAG 28727157-28727176(+) up stream 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA10 GGGTAGTAATAGAGGAGGGG 28727160-28727179(+) up stream 20 CGG CRISPRi Partial validated iPSC NA [1]

GBrowser


Links

lncRNA Function:

sgRNA Design Tool:

Reference

1. Liu SJ, Horlbeck MA, Cho SW, Birk HS, Malatesta M, et al. (2017). CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells. Science 355(6320).