CR44401
Species: Drosophila melanogaster
Position: chr3R: 20485777-20486679
Known as:
Transcript: CR44401
Sequence: Download
Description:
We identified 128 testis-specific Drosophila lncRNAs and knocked out 105 of them using an optimized three-component CRISPR/Cas9 system. Among the lncRNA knockouts, 33 (31%) exhibited a partial or complete loss of male fertility, accompanied by visual developmental defects in late spermatogenesis. In addition, six knockouts were fully or partially rescued by transgenes in a trans configuration, indicating that those lncRNAs primarily work in trans. Furthermore, gene expression profiles for five lncRNA mutants revealed that testis-specific lncRNAs regulate global gene expression, orchestrating late male germ cell differentiation.
sgRNAs
| sgRNA_ID | Sequence | Position (Chr) | Position (Lnc) | Length | PAM | Type | Validity | Cell line | Note | Ref. |
|---|---|---|---|---|---|---|---|---|---|---|
| gRNA1 | GGAGGAACTACATTCCGATC | 20485091-20485110(+) | up stream | 20 | CGG | CRISPRko | Homologous recombination efficiency: 36.36% | embryo | Knockout phenotype: no/minor phenotype | [1] |
| gRNA2 | GGACTGCTCCGTACTACGC | 20485273-20485291(+) | up stream | 19 | CGG | CRISPRko | Homologous recombination efficiency: 36.36% | embryo | Knockout phenotype: no/minor phenotype | [1] |
| gRNA3 | GGAAGGGAAAGGGTCGACCAG | 20486217-20486237(-) | gene body (near 5') | 21 | CGG | CRISPRko | Homologous recombination efficiency: 36.36% | embryo | Knockout phenotype: no/minor phenotype | [1] |
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Reference
1. Wen K, Yang L, Xiong T, Di C, Ma D, et al. (2016). Critical roles of long noncoding RNAs in Drosophila spermatogenesis. Genome Res 26(9): 1233-44.
