CTD-2245F17.3

Species: Homo sapiens

Position: chr19: 53197110-53211015

Known as:

Transcript: ENST00000601072 , ENST00000597550

Sequence: Download

Description:

We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). We classified lncRNA genes as hits if their combined phenotype effect size and P value (referred to here as “screen score”) exceeded a consistent threshold applied to each screen corresponding to an empirical FDR of 5%. Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth modifying function exclusively in one cell type.



sgRNAs

sgRNA_ID Sequence Position (Chr) Position (Lnc) Length PAM Type Validity Cell line Note Ref.
sgRNA1 CGCTCGCAGCGGAGACTGGG 53197072-53197091(-) up stream 20 CGG CRISPRi Partial validated iPSC NA [1]
sgRNA2 GCAACGTCAGAATTTACCTG 53197260-53197279(-) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA3 AGACCCGGGGGATGGAAGAA 53197192-53197211(-) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA4 AGCGGAGACTGGGCGGTCTG 53197065-53197084(-) up stream 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA5 AAAGACCAGATTCAGGAAGT 53197331-53197350(-) gene body (near 5') 20 AGG CRISPRi Partial validated iPSC NA [1]
sgRNA6 TTTCTTCCCGCCACGCGGGA 53197414-53197433(+) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA7 TAGGGACCCCTCCAGACCCG 53197205-53197224(-) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA8 TAGGCAGCAAAAGACACGAA 53197378-53197397(-) gene body (near 5') 20 AGG CRISPRi Partial validated iPSC NA [1]
sgRNA9 CTACAGAAAACCGCTCGCAG 53197083-53197102(-) up stream 20 CGG CRISPRi Partial validated iPSC NA [1]
sgRNA10 TTAGAGCGACTTTAACCCAA 53197307-53197326(-) gene body (near 5') 20 AGG CRISPRi Partial validated iPSC NA [1]

GBrowser


Links

lncRNA Function:

sgRNA Design Tool:

Reference

1. Liu SJ, Horlbeck MA, Cho SW, Birk HS, Malatesta M, et al. (2017). CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells. Science 355(6320).