CYTOR
Species: Homo sapiens
Position: chr2: 87455426-87521518
Known as: CYTOR , ENSG00000222041
Transcript: NR_024205 , NR_024204 , NR_146461 , NR_024206 , NR_146460 , ENST00000414584 , ENST00000331944 , ENST00000423846
Sequence: Download
Description:
CYTOR was significantly up-regulated in CRC samples and associated with poor prognosis, promoting proliferation and metastasis in vitro and in vivo. NCL and Sam68 could recognize their specific motifs and directly bind to EXON1 of CYTOR. Moreover, EXON1 was the key functional site mediating the interaction of CYTOR with NCL and Sam68. NCL and Sam68 functioned as oncogenes to promote CRC progression. Furthermore, we confirmed that the heterotrimeric complex of CYTOR, NCL and Sam68 activated the NF-kB pathway and EMT to contribute to CRC progression.
sgRNAs
| sgRNA_ID | Sequence | Position (Chr) | Position (Lnc) | Length | PAM | Type | Validity | Cell line | Note | Ref. |
|---|---|---|---|---|---|---|---|---|---|---|
| sgRNA1 | GGGCTCAGGCACCGCTTGTC | 87455563-87455582(-) | gene body (near 5') | 20 | TGG | CRISPRko | Experimental validated | RKO | NA | [1] |
| sgRNA2 | TTAGTCGTGTGTACATCATT | 87455480-87455499(+) | gene body (near 5') | 20 | GGG | CRISPRko | Experimental validated | RKO | NA | [1] |
| sgRNA3 | ATCTTCACAGCACAGTTCCT | 87455597-87455616(+) | gene body (near 5') | 20 | GGG | CRISPRko | Experimental validated | RKO | NA | [1] |
| sgRNA4 | GAGGCCTCTGCATTTGCGGG | 87521380-87521399(+) | gene body (near 3') | 20 | TGG | CRISPRko | Experimental validated | RKO | NA | [1] |
| sgRNA5 | ATTTTGGTCATGGGCTGGTC | 87521415-87521434(+) | gene body (near 3') | 20 | TGG | CRISPRko | Experimental validated | RKO | NA | [1] |
| sgRNA6 | TGTTGCCCGCCGATCACAGC | 87521303-87521322(+) | gene body (near 3') | 20 | CGG | CRISPRko | Experimental validated | RKO | NA | [1] |
GBrowser
Links
Reference
1. Wang X, Yu H, Sun W, Kong J, Zhang L, et al. (2018). The long non-coding RNA CYTOR drives colorectal cancer progression by interacting with NCL and Sam68. Mol Cancer 17(1): 110.
