HOXD-AS1
Species: Homo sapiens
Position: chr2: 176164050-176188958
Known as: HAGLR , ENSG00000224189
Transcript: NR_033979 , NR_110466 , NR_110465 , NR_110460 , NR_110459 , NR_110461 , NR_110468 , NR_110464 , NR_110467 , NR_110463 , NR_110462 , NR_110458 , ENST00000452365 , ENST00000416928 , ENST00000642267 , ENST00000643050 , ENST00000644334 , ENST00000645228 , ENST00000417086 , ENST00000436126 , ENST00000456876 , ENST00000425005 , ENST00000549329 , ENST00000546798 , ENST00000552156 , ENST00000447538 , ENST00000413969 , ENST00000547207
Sequence: Download
Description:
Knockdown of the lncRNA HOXD-AS1 using siRNAs did not affect the expression of its neighboring gene (HOXD1). However, dCas9-KRAB-mediated targeting of HOXD-AS1 resulted in strong repression of HOXD1, as well.
sgRNAs
| sgRNA_ID | Sequence | Position (Chr) | Position (Lnc) | Length | PAM | Type | Validity | Cell line | Note | Ref. |
|---|---|---|---|---|---|---|---|---|---|---|
| sgRNA1 | GGTCGCGACGGCTCTCCTC | 176188474-176188492(+) | gene body (near 5') | 19 | GGG | CRISPRi | Experimental validated | A549 | NA | [1] |
| sgRNA2 | GCGAGGAGCGGCGCGCCGAC | 176188556-176188575(+) | gene body (near 5') | 20 | CGG | CRISPRi | Experimental validated | A549 | NA | [1] |
| sgRNA3 | GTGGCGCTGGCCGGCCAA | 176188600-176188617(-) | gene body (near 5') | 18 | TGG | CRISPRi | Experimental validated | A549 | NA | [1] |
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Links
Reference
1. Goyal A, Myacheva K, Groß M, Klingenberg M, Duran Arqué B, et al. (2017). Challenges of CRISPR/Cas9 applications for long non-coding RNA genes. Nucleic Acids Res 45(3): e12.
