Hottip
Species: Mus musculus
Position: chr6: 52262774-52267603
Known as: Hottip , ENSMUSG00000055408
Transcript: NR_110441 , NR_110442 , ENSMUST00000141300 , ENSMUST00000152875
Sequence: Download
Description:
Hottip expression is required for activation of the 5' Hoxa genes (Hoxa13 and Hoxa10/11) and for retaining Mll1 at the 5' end of Hoxa. Moreover, we demonstrate that artificially inducing Hottip expression is sufficient to activate the 5' Hoxa genes and that Hottip RNA binds to the 5' end of Hoxa. By engineering premature transcription termination, we show that it is the Hottip lncRNA molecule itself, not just Hottip transcription that is required to maintain active expression of posterior Hox genes. Our data show a direct role for a lncRNA molecule in regulating the expression of developmentally-regulated mRNA genes in cis.
sgRNAs
| sgRNA_ID | Sequence | Position (Chr) | Position (Lnc) | Length | PAM | Type | Validity | Cell line | Note | Ref. |
|---|---|---|---|---|---|---|---|---|---|---|
| sgRNA1 | CTCCGAGAGTCTCCGAGAAT | 52262822-52262841(-) | gene body (near 5') | 20 | TGG | CRISPRko;CRISPRki | Experimental validated | ESCs;14fp | NA | [1] |
| sgRNA2 | CTCGAGGGCAGTTTACATAC | 52262843-52262862(+) | gene body (near 5') | 20 | AGG | CRISPRko | Experimental validated | ESCs;14fp | NA | [1] |
| sgRNA3 | GGCCCACTTACTCAGTTTCC | 52266844-52266863(-) | gene body (near 3') | 20 | AGG | CRISPRko | Experimental validated | ESCs;14fp | NA | [1] |
| sgRNA4 | CACTCCCTCCCGCTTTGTAC | 52266887-52266906(+) | gene body (near 3') | 20 | AGG | CRISPRko | Experimental validated | ESCs;14fp | NA | [1] |
| sgRNA5 | GCAGTAAGAAGGTAAACTCG | 52262646-52262665(-) | up stream | 20 | GGG | CRISPRa | Experimental validated | ESCs;14fp | NA | [1] |
| sgRNA6 | TCTCCTGACTTTAGCGGTCC | 52262605-52262624(+) | up stream | 20 | TGG | CRISPRa | Experimental validated | ESCs;14fp | NA | [1] |
| sgRNA7 | TACCCAGGACCGCTAAAGTC | 52262611-52262630(-) | up stream | 20 | AGG | CRISPRa | Experimental validated | ESCs;14fp | NA | [1] |
| sgRNA8 | GGATCAGGGAAGGTTTTATT | 52262547-52262566(-) | up stream | 20 | GGG | CRISPRa | Experimental validated | ESCs;14fp | NA | [1] |
| sgRNA9 | GGGATCAGGGAAGGTTTTAT | 52262548-52262567(-) | up stream | 20 | TGG | CRISPRa | Experimental validated | ESCs;14fp | NA | [1] |
GBrowser
Links
Reference
1. Pradeepa MM, McKenna F, Taylor GC, Bengani H, Grimes GR, et al. (2017). Psip1/p52 regulates posterior Hoxa genes through activation of lncRNA Hottip. PLoS Genet 13(4): e1006677.
