LINC00441
Species: Homo sapiens
Position: chr13: 48296512-48303661
Known as: RB1-DT , ENSG00000231473
Transcript: NR_046414 , ENST00000433480
Sequence: Download
Description:
LINC00441 arises from the bidirectional promoter of the tumor suppressor gene RB1 and its knockdown using siRNAs in A549 cells did not impact RB1 expression. However, dCas9-KRAB mediated knockdown of LINC00441 using three independent sgRNAs targeting its promoter strongly reduced RB1 mRNA as well as protein expression in these cells.
sgRNAs
| sgRNA_ID | Sequence | Position (Chr) | Position (Lnc) | Length | PAM | Type | Validity | Cell line | Note | Ref. |
|---|---|---|---|---|---|---|---|---|---|---|
| sgRNA1 | GCTGGTCGGTGCGCGGGCT | 48303636-48303654(-) | gene body (near 5') | 19 | GGG | CRISPRi | Experimental validated | A549 | NA | [1] |
| sgRNA2 | GTTCCCCACAGACGCCGGC | 48303661-48303679(+) | gene body (near 5') | 19 | GGG | CRISPRi | Experimental validated | A549 | NA | [1] |
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Links
Reference
1. Goyal A, Myacheva K, Groß M, Klingenberg M, Duran Arqué B, et al. (2017). Challenges of CRISPR/Cas9 applications for long non-coding RNA genes. Nucleic Acids Res 45(3): e12.
