NIPBL-AS1
Species: Homo sapiens
Position: chr5: 36871360-36876694
Known as: NIPBL-DT
Transcript: NR_046262
Sequence: Download
Description:
NIPBL-AS1 is transcribed upstream and antisense to NIPBL, the cohesin-loader protein that is found to be mutated in >60% of the cases of Cornelia de Lange syndrome (CdLS). NIPBL-AS1 originates from a promoter shared with NIPBL and has a co-regulation by a distal enhancer. Deletion of the enhancer by CRISPR leads to reduced expression of NIPBL and NIPBL-AS1 but also of dysregulated NIPBL target genes in CdLS patient cells. NIPBL-AS1 itself has no role for NIPBL expression, but its transcription reduces the expression of the NIPBL gene and vice versa, thus revealing an interesting mechanism for the fine-tuning of expression levels.
sgRNAs
| sgRNA_ID | Sequence | Position (Chr) | Position (Lnc) | Length | PAM | Type | Validity | Cell line | Note | Ref. |
|---|---|---|---|---|---|---|---|---|---|---|
| sgRNA1 | ATAGAGAACGGTGGAACAA | 36875721-36875739(+) | gene body (near 5') | 19 | TGG | CRISPRi | Experimental validated | HEK293T | NA | [1] |
| sgRNA2 | TCCAGGAAAATAAAGACAG | 36876472-36876490(+) | gene body (near 5') | 19 | AGG | CRISPRi | Experimental validated | HEK293T | NA | [1] |
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Reference
1. Zuin J, Casa V, Pozojevic J, Kolovos P, van den Hout MCGN, et al. (2017). Regulation of the cohesin-loading factor NIPBL: Role of the lncRNA NIPBL-AS1 and identification of a distal enhancer element. PLoS Genet 13(12): e1007137.
