PVT1
Species: Homo sapiens
Position: chr8: 127794532-128101253
Known as: PVT1 , ENSG00000249859
Transcript: NR_003367 , ENST00000513868 , ENST00000521951
Sequence: Download
Description:
The PVT1 promoter has a tumor-suppressor function that is independent of PVT1 lncRNA. CRISPR interference of PVT1 promoter enhances breast cancer cell competition and growth in vivo. The promoters of the PVT1 and the MYC oncogenes, located 55 kb apart on chromosome 8q24, compete for engagement with four intragenic enhancers in the PVT1 locus, thereby allowing the PVT1 promoter to regulate pause release of MYC transcription. PVT1 undergoes developmentally regulated monoallelic expression, and the PVT1 promoter inhibits MYC expression only from the same chromosome via promoter competition. Cancer genome sequencing identifies recurrent mutations encompassing the human PVT1 promoter, and genome editing verified that PVT1 promoter mutation promotes cancer cell growth. These results highlight regulatory sequences of lncRNA genes as potential disease-associated DNA elements.
sgRNAs
| sgRNA_ID | Sequence | Position (Chr) | Position (Lnc) | Length | PAM | Type | Validity | Cell line | Note | Ref. |
|---|---|---|---|---|---|---|---|---|---|---|
| sgRNA1 | CGGGTTGCCCGTGACGTCA | 127794501-127794519(-) | up stream | 19 | CGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA2 | CCTCCGGGCAGAGCGCGTG | 127794532-127794550(+) | gene body (near 5') | 19 | TGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA3 | CCGGGCAGAGCGCGTGTGG | 127794535-127794553(+) | gene body (near 5') | 19 | CGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA4 | GTGTGGCGGCCGAGCACAT | 127794548-127794566(+) | gene body (near 5') | 19 | GGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA5 | GGCCGAGCACATGGGCCCG | 127794555-127794573(+) | gene body (near 5') | 19 | CGG | CRISPRi;CRISPRedit | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA6 | CGAGACTTGGCCCTGAGTA | 127794774-127794792(+) | gene body (near 5') | 19 | GGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA7 | CACAGCGCATCCCTACTCA | 127794787-127794805(-) | gene body (near 5') | 19 | GGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA8 | GGGATGCGCTGTGAGTAGT | 127794793-127794811(+) | gene body (near 5') | 19 | CGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA9 | GTAGTCGGACGGAGGAAAG | 127794807-127794825(+) | gene body (near 5') | 19 | GGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA10 | CCGAATGGCCGGTGCATAG | 127794937-127794955(+) | gene body (near 5') | 19 | AGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA11 | ATTAGGAGAAAAACCTTCC | 127795899-127795917(+) | gene body (near 5') | 19 | CGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA12 | CCTTCCCGGAAGCTGCAGA | 127795912-127795930(+) | gene body (near 5') | 19 | AGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA13 | AAATACAGAATCCGTGTCT | 127795936-127795954(+) | gene body (near 5') | 19 | GGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA14 | GTGTCTGGGAGAAACCTCG | 127795949-127795967(+) | gene body (near 5') | 19 | TGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA15 | TGGGAGAAACCTCGTGGCC | 127795954-127795972(+) | gene body (near 5') | 19 | TGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA16 | TAATGGAGACCAGGCCACG | 127795966-127795984(-) | gene body (near 5') | 19 | AGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA17 | ATCTCAAATAATGGAGACC | 127795974-127795992(-) | gene body (near 5') | 19 | AGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA18 | GCCGAAGCTTTAGACCACG | 127796023-127796041(-) | gene body (near 5') | 19 | AGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA19 | GAATAAGCCACTCTTAACC | 127796149-127796167(-) | gene body (near 5') | 19 | AGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA20 | TCTGCACTCAAGATAGATG | 127796177-127796195(-) | gene body (near 5') | 19 | AGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA21 | GCGAGCCGCCGTGACGTCA | 127794489-127794507(+) | up stream | 19 | CGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA22 | CGTCACGGCGGCTCGCGCT | 127794486-127794504(-) | up stream | 19 | CGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA23 | CGGCTCGCGCTCGGGCCTC | 127794479-127794497(-) | up stream | 19 | GGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA24 | GAGGACGCACGCGGCGGGC | 127794431-127794449(+) | up stream | 19 | CGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA25 | GGCCCGCCGCGTGCGTCCT | 127794432-127794450(-) | up stream | 19 | CGG | CRISPRi | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA26 | GGACCGAGGACGCACGCGGC | 127794426-127794445(+) | up stream | 20 | GGG | CRISPRa | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA27 | GCCGGGACCGAGGACGCACG | 127794422-127794441(+) | up stream | 20 | CGG | CRISPRa | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA28 | GCCTGCTTCCCGGCAGCGC | 127794355-127794373(-) | up stream | 19 | AGG | CRISPRa | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA29 | GGGCGACTGCGGCCGCCAA | 127794290-127794308(-) | up stream | 19 | GGG | CRISPRa | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA30 | GCCGCCACACGCGCTCTGCC | 127794538-127794557(-) | gene body (near 5') | 20 | CGG | CRISPRedit | Experimental validated | MDA-MB-231;MCF-7 | NA | [1] |
| sgRNA32 | TCTCCAGAAGGACAGAATAA | 128096511-128096530(+) | gene body (near 3') | 20 | CGG | CRISPRi | Experimental validated | T24;5637 | NA | [2] |
| sgRNA33 | AAAAGAATTTAATAGACACG | 128101227-128101246(-) | gene body (near 3') | 20 | AGG | CRISPRi | Experimental validated | T24;5637 | NA | [2] |
| sgRNA34 | TTGGTGGGGCTTGTGAATC | 128096540-128096559(-) | gene body (near 3') | 19 | TGG | CRISPRi | Experimental validated | T24;5637 | NA | [2] |
| sgRNA35 | ACGAGGCCGGCCACGCCACG | 128101210-128101229(-) | gene body (near 3') | 20 | TGG | CRISPRi | Experimental validated | T24;5637 | NA | [2] |
| sgRNA36 | GATTCACAAGCCCCACCAAG | 128096541-128096560(+) | gene body (near 3') | 20 | AGG | CRISPRi | Experimental validated | T24;5637 | NA | [2] |
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Links
Reference
1. Cho SW, Xu J, Sun R, Mumbach MR, Carter AC, et al. (2018). Promoter of lncRNA Gene PVT1 Is a Tumor-Suppressor DNA Boundary Element. Cell 173(6): 1398-1412.e22.
2. Peng L, Pan P, Chen J, Yu X, Wu J, et al. (2018). A tetracycline-inducible CRISPR/Cas9 system, targeting two long non-coding RNAs, suppresses the malignant behavior of bladder cancer cells. Oncol Lett 16(4): 4309-4316.