RP11-108L7.15

Species: Homo sapiens

Position: chr10: 101060028-101061005

Known as:

Transcript: ENST00000609242

Sequence: Download

Description:

We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). We classified lncRNA genes as hits if their combined phenotype effect size and P value (referred to here as “screen score”) exceeded a consistent threshold applied to each screen corresponding to an empirical FDR of 5%. Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth modifying function exclusively in one cell type.



sgRNAs

sgRNA_ID Sequence Position (Chr) Position (Lnc) Length PAM Type Validity Cell line Note Ref.
sgRNA1 AGCCACGTAGGCGCCATCCC 101060888-101060907(-) gene body (near 5') 20 TGG CRISPRi Partial validated iPSC NA [1]
sgRNA2 CCCTGGCCAGAGGGGTTCTG 101060871-101060890(-) gene body (near 5') 20 AGG CRISPRi Partial validated iPSC NA [1]
sgRNA3 AGAAAGGGCGGAGCAAACTC 101060695-101060714(-) gene body (near 5') 20 TGG CRISPRi Partial validated iPSC NA [1]
sgRNA4 TCAGGTCGACACTTATTCCA 101060644-101060663(+) gene body (near 5') 20 AGG CRISPRi Partial validated iPSC NA [1]
sgRNA5 AGAGTCAGAGACCTCTCCTT 101060537-101060556(+) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA6 GGCCAGGGATGGCGCCTACG 101060883-101060902(+) gene body (near 5') 20 TGG CRISPRi Partial validated iPSC NA [1]
sgRNA7 GGGCCAAGCCCATTCATTCA 101060918-101060937(-) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA8 CAGGGGCTCCCGAGCCACGT 101060900-101060919(-) gene body (near 5') 20 AGG CRISPRi Partial validated iPSC NA [1]
sgRNA9 CAAGGGCAGACACTCTCTCC 101060841-101060860(-) gene body (near 5') 20 CGG CRISPRi Partial validated iPSC NA [1]
sgRNA10 TGGCCAGAGGGGTTCTGAGG 101060868-101060887(-) gene body (near 5') 20 AGG CRISPRi Partial validated iPSC NA [1]

GBrowser


Links

lncRNA Function:

sgRNA Design Tool:

Reference

1. Liu SJ, Horlbeck MA, Cho SW, Birk HS, Malatesta M, et al. (2017). CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells. Science 355(6320).