RP11-146F11.1

Species: Homo sapiens

Position: chr16: 30697706-30699058

Known as:

Transcript: ENST00000568500 , ENST00000564775

Sequence: Download

Description:

We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). We classified lncRNA genes as hits if their combined phenotype effect size and P value (referred to here as “screen score”) exceeded a consistent threshold applied to each screen corresponding to an empirical FDR of 5%. Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth modifying function exclusively in one cell type.



sgRNAs

sgRNA_ID Sequence Position (Chr) Position (Lnc) Length PAM Type Validity Cell line Note Ref.
sgRNA1 GGGCGTGGGACGAGTAGTCG 30698823-30698842(+) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA2 TGCGTGGGCTCGGGTTCTGG 30698985-30699004(+) gene body (near 5') 20 CGG CRISPRi Partial validated iPSC NA [1]
sgRNA3 CCCGCGACCCTTGTGTGCGT 30698970-30698989(+) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA4 GGGTTCTGGCGGGAGCCCCT 30698996-30699015(+) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA5 TTCGCGGTCGGGTGATGGGG 30698913-30698932(+) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA6 ACCCGAGCCCACGCACACAA 30698980-30698999(-) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA7 CCAACCCAGTCACCCCTCAG 30698781-30698800(+) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA8 CCCATAGAGACAGACTGAGG 30698628-30698647(+) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA9 CTAGCCGGGGGACCAGGACC 30698862-30698881(+) gene body (near 5') 20 TGG CRISPRi Partial validated iPSC NA [1]
sgRNA10 GTCCCCCGGCTAGATGTGGT 30698855-30698874(-) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]

GBrowser


Links

lncRNA Function:

sgRNA Design Tool:

Reference

1. Liu SJ, Horlbeck MA, Cho SW, Birk HS, Malatesta M, et al. (2017). CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells. Science 355(6320).