RP11-649E7.7

Species: Homo sapiens

Position: chr14: 49629290-49629890

Known as:

Transcript: ENST00000556657

Sequence: Download

Description:

We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). We classified lncRNA genes as hits if their combined phenotype effect size and P value (referred to here as “screen score”) exceeded a consistent threshold applied to each screen corresponding to an empirical FDR of 5%. Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth modifying function exclusively in one cell type.



sgRNAs

sgRNA_ID Sequence Position (Chr) Position (Lnc) Length PAM Type Validity Cell line Note Ref.
sgRNA1 ACAGTTTGCTAATGTTACGC 49629841-49629860(-) gene body (near 5') 20 TGG CRISPRi Partial validated iPSC NA [1]
sgRNA2 TACATTTTTGGGGCCTGGTG 49629456-49629475(+) gene body (near 3') 20 TGG CRISPRi Partial validated iPSC NA [1]
sgRNA3 GTATTATATTTTTAAGCACT 49629810-49629829(+) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA4 ATTTTTACATTTTTGGGGCC 49629451-49629470(+) gene body (near 3') 20 TGG CRISPRi Partial validated iPSC NA [1]
sgRNA5 GAAATATAATACACTTGGCT 60649499-60649518(-) up stream 20 TGG CRISPRi Partial validated iPSC NA [1]
sgRNA6 GATTGCAGTGAGTCCAGCCT 49629619-49629638(+) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA7 TGCAATCACGTGAACCCGTG 49629592-49629611(+) gene body (near 5') 20 AGG CRISPRi Partial validated iPSC NA [1]
sgRNA8 GCTTAAAAATATAATACACT 49629807-49629826(-) gene body (near 5') 20 TGG CRISPRi Partial validated iPSC NA [1]
sgRNA9 TCCCAGGTACCCAGGCACTT 49629402-49629421(-) gene body (near 3') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA10 AAGTAGAGAGCATATATTCA 49629879-49629898(+) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]

GBrowser


Links

lncRNA Function:

sgRNA Design Tool:

Reference

1. Liu SJ, Horlbeck MA, Cho SW, Birk HS, Malatesta M, et al. (2017). CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells. Science 355(6320).