SPRIGHTLY
Species: Homo sapiens
Position: chr5: 142317619-142318322
Known as: SPRY4-IT1
Transcript: NR_131221
Sequence: Download
Description:
The aberrant expression of SPRIGHTLY lncRNA, encoded within the drosophila gene homolog Sprouty-4 intron, is correlated with a variety of cancers, including human melanomas. SHAPE-seq and dChIRP revealed that SPRIGHTLY RNA secondary structure has a core pseudonotted domain. This lncRNA interacts with the intronic regions of six pre-mRNAs: SOX5, SMYD3, SND1, MEOX2, DCTN6, andRASAL2, all of which have cancer-related functions. Hemizygous knockout of SPRIGHTLY by CRISPR/Cas9 in melanoma cells significantly decreases SPRIGHTLY lncRNA levels, simultaneously decreases the levels of its interacting pre-mRNA molecules, and decreases anchorage-independent growth rate of cells and the rate of in vivo tumor growth in mouse xenografts. These results provide the first demonstration of an lncRNA's three-dimensional coordinating role in facilitating cancer-related gene expression in human melanomas.
sgRNAs
| sgRNA_ID | Sequence | Position (Chr) | Position (Lnc) | Length | PAM | Type | Validity | Cell line | Note | Ref. |
|---|---|---|---|---|---|---|---|---|---|---|
| sgRNA1 | GCTCCCTGCTTCCCCTGCA | 142318620-142318638(+) | up stream | 19 | AGG | CRISPRko | Experimental validated | A375 | paried with sgRNA2 | [1] |
| sgRNA2 | TCATGTTATCATTGTCCGG | 142317425-142317443(-) | down stream | 19 | GGG | CRISPRko | Experimental validated | A375 | paried with sgRNA1 | [1] |
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Links
Reference
1. Lee B, Sahoo A, Marchica J, Holzhauser E, Chen X, et al. (2017). The long noncoding RNA SPRIGHTLY acts as an intranuclear organizing hub for pre-mRNA molecules. Sci Adv 3(5): e1602505.
