TNKS2-AS1

Species: Homo sapiens

Position: chr10: 91782935-91798196

Known as:

Transcript: ENST00000432246

Sequence: Download

Description:

We developed a CRISPR interference (CRISPRi) platform targeting 16,401 lncRNA loci in seven diverse cell lines, including six transformed cell lines and human induced pluripotent stem cells (iPSCs). We classified lncRNA genes as hits if their combined phenotype effect size and P value (referred to here as “screen score”) exceeded a consistent threshold applied to each screen corresponding to an empirical FDR of 5%. Large-scale screening identified 499 lncRNA loci required for robust cellular growth, of which 89% showed growth modifying function exclusively in one cell type.



sgRNAs

sgRNA_ID Sequence Position (Chr) Position (Lnc) Length PAM Type Validity Cell line Note Ref.
sgRNA1 AGGCGGCGCGAAGGGTTTGT 91798302-91798321(-) up stream 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA2 TGTGGGAAATGTGAGGCGAG 91798285-91798304(-) up stream 20 AGG CRISPRi Partial validated iPSC NA [1]
sgRNA3 GCAGGTTTCGGCTAGCGAGG 91798319-91798338(-) up stream 20 CGG CRISPRi Partial validated iPSC NA [1]
sgRNA4 CCCACCGCAAAGATGGCGGT 91798232-91798251(+) up stream 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA5 AAGGGTTTGTGGGAAATGTG 91798292-91798311(-) up stream 20 AGG CRISPRi Partial validated iPSC NA [1]
sgRNA6 AGTTCAGCCTGGAAAGCCCA 91798153-91798172(+) gene body (near 5') 20 GGG CRISPRi Partial validated iPSC NA [1]
sgRNA7 CGGCTAGCGAGGCGGCGCGA 91798311-91798330(-) up stream 20 AGG CRISPRi Partial validated iPSC NA [1]
sgRNA8 GAGGCGAGAGGCTTCGGCGG 91798273-91798292(-) up stream 20 CGG CRISPRi Partial validated iPSC NA [1]
sgRNA9 TGTGAGGCGAGAGGCTTCGG 91798276-91798295(-) up stream 20 CGG CRISPRi Partial validated iPSC NA [1]
sgRNA10 AGCAGAACCCACCGCAAAGA 91798225-91798244(+) up stream 20 TGG CRISPRi Partial validated iPSC NA [1]

GBrowser


Links

lncRNA Function:

sgRNA Design Tool:

Reference

1. Liu SJ, Horlbeck MA, Cho SW, Birk HS, Malatesta M, et al. (2017). CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells. Science 355(6320).