Tsix
Species: Mus musculus
Position: chrX: 103431516-103484957
Known as: Tsix , ENSMUSG00000085715
Transcript: NR_002844 , ENSMUST00000152916 , ENSMUST00000211291
Sequence: Download
Description:
Tsix, a negative regulator of Xist, allows allele-specific analysis of X-linked gene expression. We show that we can modulate the efficiency of exon-skipping type alternative splicing in Tsix by CRISPR/Cas-mediated intron targeting.
sgRNAs
| sgRNA_ID | Sequence | Position (Chr) | Position (Lnc) | Length | PAM | Type | Validity | Cell line | Note | Ref. |
|---|---|---|---|---|---|---|---|---|---|---|
| sgRNA1 | ACAGTGGCAAAGTGCTTTCA | 103448184-103448203(+) | gene body (near 5') | 20 | GGA | CRISPRedit | Experimental validated | ESCs | NA | [1] |
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Links
Reference
1. Yue M, Ogawa Y (2018). CRISPR/Cas9-mediated modulation of splicing efficiency reveals short splicing isoform of Xist RNA is sufficient to induce X-chromosome inactivation. Nucleic Acids Res 46(5): e26.
